Spectrum of activity The tetracyclines are mainly bacteriostatic, with a broad spectrum of antimicrobial activity. The following Gram-positive and Gram-negative pathogenic bacteria are usually sensitive to tetracyclines: Staphylococcus aureus, Streptococcus pneumoniae, Listeria spp., Bacillus anthracis, Clostridium spp., Actinomyces israelii; Haemophilus influenzae, Haemophilus ducreyi, Bordetella pertussis, enterobacteria (Escherichia coli, Enterobacter spp., Enterobacter aerogenes, Klebsiella spp., Salmonella spp., Shigella spp., Yersinia pestis, Bartonella bacilliformis, Vibrio cholerae, Vibrio fetus, Rickettsia spp., Borrelia burgdorferi, Brucella spp. Mechanism of action Tetracyclines are taken up into sensitive bacterial cells by an active transport process. Once appeared within the cell, they reversibly affiliate to the 30S subunit of the ribosome in bacterial cells. 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